Regulatory
Part:BBa_K1678005:Design
Designed by: Antoine Vigouroux Group: iGEM15_Paris_Bettencourt (2015-09-16)
Promoter from BBa_J23119 followed by 4 orthogonal LoxP sites
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Spacer have been added between the LoxP sites to allow for primer binding.
Source
DNA synthesis